ZEISS TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF) MICROSCOPE The LSM 710 BIG is equipped with environmental controls (for temperature and CO2) and with two GaAsP detectors (BiG) allowing measurements of very low laser levels, therefore enabling the confocal to be used for long term live cell experiments. The upgraded ZEN software and “Smart Setup” feature allows for easy importing of fluorophore configurations, while the Definite Focus component can help to correct axial drift during long-term capture. Though fundamentally similar to the LSM 510 META capabilities, the more advanced LSM 710 META confocal microscope provides greater sensitivity and reduced background noise during acquisition. An intuitive time-lapse imaging function is useful for kinetic studies and FRAP experiments, and the laser configuration settings are suitable for FRET measurements. The specialized META detector can be used to capture detailed spectral information and customize emission filters for the elimination of signal overlap. Members of the LSM 710 confocal microscope family are popular, full-featured instruments capable of optically sectioning living or fixed specimens of varying thickness. ZEISS LASER SCANNING MICROSCOPES: LSM 710 META and LSM 710 BIG Instrument time can be scheduled on iLabs. Please check the Getting Started page to register on iLabs.
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